Tubulin Protein by PurSolutions
Tubulin Protein In Biomedical Research
- highly conserved superfamily of cytoskeletal proteins
- alpha and beta tubulin heterodimers polymerize into microtubules, a major component of the cytoskeleton
- nucleation primarily occurs at the centrosome and other microtubule organizing centers (MTOCs)
- inherent GTPase activity drives microtubule dynamic instability
- substrate for kinesin and dynein motor proteins important for intracellular transport
- primary structural component of the mitotic spindle, cilia, and flagella
- essential for cell morphology, mitosis, meiosis, and other cellular functions
- tubulin mutations cause tubulinopathy and neuronal disorders
Cryopreserved
Cryopreserved Tubulin
Catalog Number: 032005 Isolated to >99% purity by polymerization cycling and cryopreserved for optimal performance.
Cycled Tubulin™ or cryopreserved tubulin is isolated by cycling bovine brain homogenate through conditions that promote tubulin polymerization/depolymerization in high salt buffers. The resulting protein is >99% pure and cryopreserved at 20 mg/ml in 80 mM PIPES, 1 mM MgCl2, and 1 mM EGTA (pH 6.8).
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Our Best Seller! The Most Active And Soluble Tubulin Protein On The Market: $150/mg
Citations
Ross T, et al. Controlling Organization And Forces In Active Matter Through Optically Defined Boundaries. Nature. 2019. Link Sandate R, et al. An Allosteric Network In Spastin Couples Multiple Activities Required For Microtubule Severing. Nature Structural & Molecular Biology. 2019. Link Jiang, et al. Interplay between the Kinesin and Tubulin Mechanochemical Cycles Underlies Microtubule Tip Tracking by the Non-motile Ciliary Kinesin Kif7. Dev Cell. 2019. LinkLyophilized
Lyophilized Tubulin
Catalog Number: 142001 Isolated to >99% by selective precipitation and lyophilized for storage at room temperature.
Lyophilized Tubulin is isolated by selective precipitation from bovine brain homogenate. The resulting protein is >99% pure and lyophilized. When reconstituted with ultrapure water to 40 mg/ml, the buffer conditions are 10 mM Sodium Phosphate, 0.5 mM MgCl2, 0.1 mM GTP, and 0.25 M Trehalose (pH 7.0)
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Tubulin Protein Stored And Shipped At Room Temperature: $105/mg
Alexa Fluor® 488
Labeled Tubulin-Alexa Fluor® 488
Catalog Number: 048805 99% pure tubulin protein conjugated with a green fluorescent dye.
Labeled Tubulin-Alexa Fluor® 488 is generated by covalently linking the dye to random tubulin surface lysines in its polymerized form. The final product is cryopreserved at 20 mg/ml in 50 mM K-Glutamate and 0.5 mM MgCl2 (pH=7.0), and is commonly visualized with a FITC filter set with maximum excitation/emission wavelengths of 494/517 nm.
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The Brightest Green Tubulin Protein On The market: $295/0.1mg
Alexa Fluor® 594
Labeled Tubulin-Alexa Fluor® 594
Catalog Number: 059405 99% pure tubulin protein conjugated with a red fluorescent dye.
Labeled Tubulin-Alexa Fluor® 594 is generated by covalently linking the dye to random tubulin surface lysines in its polymerized form. The final product is cryopreserved at 20 mg/ml in 50 mM K-Glutamate and 0.5 mM MgCl2 (pH=7.0), and is commonly visualized with a Texas Red filter set with maximum excitation/emission wavelengths of 590/617 nm.
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The Brightest Red Tubulin Protein On The market: $295/0.1mg
Alexa Fluor® 647
Labeled Tubulin-Alexa Fluor® 647
Catalog Number: 064705 99% pure tubulin protein conjugated with a far red fluorescent dye.
Labeled Tubulin-Alexa Fluor® 647 is generated by covalently linking the dye to random tubulin surface lysines in its polymerized form. The final product is cryopreserved at 20 mg/ml in 50 mM K-Glutamate and 0.5 mM MgCl2 (pH=7.0), and is commonly visualized with a Cy5 filter set with maximum excitation/emission wavelengths of 651/672 nm.
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The Brightest Far Red Tubulin Protein On The market: $295/0.1mg
Citations
Zehr EA, et al. Katanin Grips the β-Tubulin Tail through an Electropositive Double Spiral to Sever Microtubules. Dev. Cell 2019. Link Ross T, et al. Controlling Organization And Forces In Active Matter Through Optically Defined Boundaries. Nature. 2019. LinkFarhadi L, et al. Active Self-Organization of Actin-Microtubule Composite Self-Propelled Rods. Front Phys. 2018. Link
Cy5
Labeled Tubulin-Cy5
Catalog Number: 065005 99% pure tubulin protein conjugated with a far red fluorescent dye.
Labeled Tubulin-Cy5 is generated by covalently linking the dye to random tubulin surface lysines in its polymerized form. The final product is cryopreserved at 20 mg/ml in 50 mM K-Glutamate and 0.5 mM MgCl2 (pH=7.0), and is commonly visualized with a Cy5 filter set with maximum excitation/emission wavelengths of 649/67 nm.
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The Most Photostable Tubulin Protein On The Market: $295/0.1mg
Biotin-XX
Labeled Tubulin-Biotin-XX
Catalog Number: 033305 99% pure tubulin protein conjugated with a long-chain biotin derivative.
Labeled Tubulin-Biotin-XX is generated by covalently linking the biomolecule to random tubulin surface lysines in its polymerized form. The final product is cryopreserved at 20 mg/ml in 50 mM K-Glutamate and 0.5 mM MgCl2 (pH=7.0), and demonstrates an affinity toward streptavidin-coated beads and streptavidin-coated coverslips.
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Tubulin Protein With A Strong Affinity For Streptavidin: $295/0.1mg
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