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Customer Satisfaction Rating 4.8 out of 5 stars
“The Cycled Tubulin from PurSolutions is qualitatively as good as the one that we make in the lab. It is usable for live polymerization assay. To our knowledge, no other company managed to achieve this before and we can definitely recommend it!”
Customer Satisfaction Rating 4.8 out of 5 stars
“The (Ultra-Pure Actin) was good, slower polymerization kinetics than the too-fast Cytoskeleton Inc actin even without gel filtration. We'll order it again.”
Customer Satisfaction Rating 4.8 out of 5 stars
“Great solubility and clear labeling”
Cycled Tubulin™ (>99%)
Cycled Tubulin™ is isolated by cycling bovine brain homogenate through conditions that promote tubulin polymerization/depolymerization in high salt buffers. The resulting protein is >99% pure and cryopreserved at 20 mg/ml in 80 mM PIPES, 1 mM MgCl2, and 1 mM EGTA (pH 6.8).
Our Best Seller! The most Active and Soluble Tubulin Protein on the market: $150/mg
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Citations
Alfaro-Aco R, et al. Structural Analysis of the Role of TPX2 in Branching Microtubule Nucleation. J Cell Biol. 2017. Link
Howes SC, et al. Structural and Functional Differences between Porcine Brain and Budding Yeast Microtubules. Cell Cycle. 2018. Link
Thawani A, et al. XMAP215 Is a Microtubule Nucleation Factor That Functions Synergistically with the γ-Tubulin Ring Complex. Nat Cell Biol. 2018. Link
Schimert KI, et al. Intracellular Cargo Transport By Single-Headed Kinesin Motors. PNAS. 2019. Link
And more...
Labeled Tubulin-Alexa Fluor® 488 is generated by covalently linking the dye to random tubulin surface lysines in its polymerized form. The final product is cryopreserved at 20 mg/ml in 50 mM K-Glutamate and 0.5 mM MgCl2 (pH=7.0), and is commonly visualized with a FITC filter set with maximum excitation/emission wavelengths of 494/517 nm.
Labeled Tubulin-Alexa Fluor® 594 is generated by covalently linking the dye to random tubulin surface lysines in its polymerized form. The final product is cryopreserved at 20 mg/ml in 50 mM K-Glutamate and 0.5 mM MgCl2 (pH=7.0), and is commonly visualized with a Texas Red filter set with maximum excitation/emission wavelengths of 590/617 nm.
Labeled Tubulin-Alexa Fluor® 647 is generated by covalently linking the dye to random tubulin surface lysines in its polymerized form. The final product is cryopreserved at 20 mg/ml in 50 mM K-Glutamate and 0.5 mM MgCl2 (pH=7.0), and is commonly visualized with a Cy5 filter set with maximum excitation/emission wavelengths of 651/672 nm.
Labeled Tubulin-Biotin-XX is generated by covalently linking the biomolecule to random tubulin surface lysines in its polymerized form. The final product is cryopreserved at 20 mg/ml in 50 mM K-Glutamate and 0.5 mM MgCl2 (pH=7.0), and demonstrates an affinity toward streptavidin-coated beads and streptavidin-coated coverslips.Some of our Customers
Customers Include: