Storing and Handling Cryopreserved Tubulin

Overview


Tubulin is a labile molecule that loses activity within hours of being thawed. When handling the cryopreserved products listed below, it is strongly recommended to follow these steps. Even perceived slight differences in handling can have a significant impact on product quality. For tubulin that is stable at room temperature please refer to Lyophilized Tubulin (Cat. No. 142001).

Overview

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Tubulin is a labile molecule that loses activity within hours of being thawed. When handling the cryopreserved products listed below, it is strongly recommended to follow these steps. Even perceived slight differences in handling can have a significant impact on product quality. For tubulin that is stable at room temperature please refer to Lyophilized Tubulin (Cat. No. 142001).

Recommended Products


Unlabeled Tubulin


Labeled Tubulin


Recommended Products


Unlabeled Tubulin

Labeled Tubulin

Recommended Products


Unlabeled Tubulin

Unlabeled Tubulin

Labeled Tubulin

Protocol


1. Store at -80°C

Immediately transfer tubulin to -80°C upon receipt.

2. Thaw at 37°C

When ready to use, thaw tubulin in a 37°C water bath until ~80% thawed. Thaw to completion by flicking tube.

 NOTE :

Thawing on ice or at room temperature is NOT recommended.

3. Work on ice

Immediately place tubulin on ice after thawing. Continue to work on ice.

4. Aliquot

Aliquot tubulin into experimental-sized batches to avoid repeated freeze-thaw cycles.

5. Freeze in Liquid Nitrogen

Refreeze experimental-sized aliquots in liquid Nitrogen.

 NOTE :

Failure to flash freeze with liquid Nitrogen will result in significant reduction of activity.

6. Store at -80°C

Transfer frozen experimental aliquots to -80°C. When ready to use, thaw as directed above.

 NOTE :

Avoid repeated freeze-thaw cycles. Discard any unused portion of the experimental aliquots.

Protocol


Step 1

1. Store at -80°C

Immediately transfer tubulin to -80°C upon receipt.

Step 2

2. Thaw at 37°C

When ready to use, thaw tubulin in a 37°C water bath until ~80% thawed. Thaw to completion by flicking tube.

 NOTE :

Thawing on ice or at room temperature is NOT recommended.

Step 3

3. Work on ice

Immediately place tubulin on ice after thawing. Continue to work on ice.

Step 4

4. Aliquot

Aliquot tubulin into experimental-sized batches to avoid repeated freeze-thaw cycles.

Step 5

5. Freeze in Liquid Nitrogen

Refreeze experimental-sized aliquots in liquid Nitrogen.

 NOTE :

Failure to flash freeze with liquid Nitrogen will result in significant reduction of activity.

Step 6

6. Store at -80°C

Transfer frozen experimental aliquots to -80°C. When ready to use, thaw as directed above.

 NOTE :

Avoid repeated freeze-thaw cycles. Discard any unused portion of the experimental aliquots.

Protocol

Step 1

1. Store at -80°C

Immediately transfer tubulin to -80°C upon receipt.

Step 2

2. Thaw at 37°C

When ready to use, thaw tubulin in a 37°C water bath until ~80% thawed. Thaw to completion by flicking tube.

 NOTE :

Thawing on ice or at room temperature is NOT recommended.

Step 3

3. Work on ice

Immediately place tubulin on ice after thawing. Continue to work on ice.

Step 4

4. Aliquot

Aliquot tubulin into experimental-sized batches to avoid repeated freeze-thaw cycles.

Step 5

5. Freeze in Liquid Nitrogen

Refreeze experimental-sized aliquots in liquid Nitrogen.

 NOTE :

Failure to flash freeze with liquid Nitrogen will result in significant reduction of activity.

Step 6

6. Store at -80°C

Transfer frozen experimental aliquots to -80°C. When ready to use, thaw as directed above.

 NOTE :

Avoid repeated freeze-thaw cycles. Discard any unused portion of the experimental aliquots.