Polymerizing Actin

Overview


Monomeric G-actin polymerizes into filamentous F-actin when supplemented with KCl and MgCl2. F-actin can be stored at 4°C for several weeks with the addition of 0.05% sodium azide. For general actin handling instructions, please see “Storing and Handling Ultra-Pure Actin” or “Storing and Handling Pure Actin”.

Overview


Monomeric G-actin polymerizes into filamentous F-actin when supplemented with KCl and MgCl2. F-actin can be stored at 4°C for several weeks with the addition of 0.05% sodium azide. For general actin handling instructions, please see “Storing and Handling Ultra-Pure Actin” or “Storing and Handling Pure Actin”.

Recommended Products


Unlabeled Tubulin

Unlabeled Tubulin

Buffers

Recommended Products


Recommended Products

Unlabeled Tubulin

Buffers

Overview


Monomeric G-actin polymerizes into filamentous F-actin when supplemented with KCl and MgCl2. F-actin can be stored at 4°C for several weeks with the addition of 0.05% sodium azide. For general actin handling instructions, please see “Storing and Handling Ultra-Pure Actin” or “Storing and Handling Pure Actin”.

Recommended Products


Unlabeled Actin


Buffers


Protocol


1. Assemble

Assemble the polymerization reaction on ice by adding 1/10th volume Actin Polymer Buffer (10X) to actin at 0.4 mg/ml.

Add ATP to 1 mM.

2. Polymerize

Incubate the polymerization reaction at room temperature for 1 hour.

At this point, the F-actin can be used directly or it can be stored at 4°C for several weeks with the addition of 0.05% sodium azide.

Protocol


Step 1

1. Assemble

Assemble the polymerization reaction on ice by adding 1/10th volume Actin Polymer Buffer (10X) to actin at 0.4 mg/ml.

Add ATP to 1 mM.

Step 2

2. Polymerize

Incubate the polymerization reaction at room temperature for 1 hour.

At this point, the F-actin can be used directly or it can be stored at 4°C for several weeks with the addition of 0.05% sodium azide.

Protocol


Step 1

1. Assemble

Assemble the polymerization reaction on ice by adding 1/10th volume Actin Polymer Buffer (10X) to actin at 0.4 mg/ml.

Add ATP to 1 mM.

Step 2

2. Polymerize

Incubate the polymerization reaction at room temperature for 1 hour.

At this point, the F-actin can be used directly or it can be stored at 4°C for several weeks with the addition of 0.05% sodium azide.