1. Construct Flow Cell
Assemble a glass “flow-cell” chamber by sandwiching two strips of double stick tape between a glass slide and glass coverslip. The two tape strips should be placed ~10 mm apart to create a chamber that holds ~15 µl volume. The chamber is open on either end so that buffer can be flowed through the chamber by pipetting into one end while simultaneously wicking from the other end.
Upon starting the experiment, do not allow the chamber to dry out. Keep the volume saturated at all times.