
Overview
The motility of kinesin and/or dynein ensembles can be assessed in microtubule gliding assays wherein glass imaging chambers, or flow cells, are coated with motor proteins and infused with microtubule-containing solutions. For a guide to polymerizing fluorescent and/or biotinylated microtubules, visit the protocols Generating GMPCPP-Stabilized Microtubules and Generating Taxol-Stabilized Microtubules. Segmented or polarity-marked microtubules can also be used to assess motor directionality.