Microtubule Co-Pelleting Assay

Microtubule co-pelleting assay graphical abstract

Overview

At relatively high centripetal forces, polymerized microtubules will sediment while unpolymerized tubulin protein will remain suspended in solution. This biophysical property can be leveraged in probing for microtubule-associated proteins and assessing tubulin polymerization competency (when stabilizing factors such as taxol are excluded).  This protocol outlines how to fractionate tubulin protein solutions by centrifugation and probe the resulting pellet and supernatant fractions.

Protocol