LYOPHILIZED TUBULIN (>99%)
Catalog Number: 142001
Source: Bovine Brain
Material Lyophilized Tubulin is isolated by selective precipitation from bovine brain homogenate by an adaptation of the method of Andreu (2007) and lyophilized by an adaptation of the method of Dráberová et al. (2010). The resulting product is >99% pure (Figure 1) and polymerization competent (Figure 2). Lyophilized Tubulin is supplied as a white powder. When reconstituted with ultrapure water to 40 mg/ml, the buffer conditions are 10 mM Sodium Phosphate, 0.5 mM MgCl2, 0.1 mM GTP, and 0.25 M Trehalose, pH 7.0. Note that 1 mg tubulin protein is supplied as 3 mg powder, and reconstitution/dilution should be based on tubulin protein concentration.
Activity and Applications Lyophilized Tubulin will polymerize into microtubules when supplemented with GTP, warmed to 37˚C, and kept above its critical concentration. Lyophilized Tubulin is suitable for use in a variety of cell-free experimental applications, and can be combined with fluorescent or biotinylated tubulin proteins in generating microtubules in vitro. Visit our protocols page for common microtubule polymerization protocols, including the generation of short, rigid microtubules stabilized by GMPCPP or long, flexible microtubules stabilized by taxol.
- structural analysis by X-ray crystallography and electron microscopy
- drug discovery by high-throughput screening
- in vitro biochemical and biophysical approaches
- kinase phosphorylation assays
TUBULIN PROTEIN STORED AND SHIPPED AT ROOM TEMPERATURE
Figure 2: Lyophilized Tubulin is polymerization-competent. Optical density (340 nm) of Lyophilized Tubulin at 5 mg/ml in Tubulin PEM Buffer (Cat. No. 032002; 80 mM PIPES, 1 mM EGTA, and 1 mM MgCl2, pH 6.8) supplemented with 1 mM GTP and 20% glycerol at 37°C. Distinct nucleation and polymerization phases are evident.