LYOPHILIZED TUBULIN (>99%)
Catalog Number: 142001
Source: Bovine Brain
Lyophilized Tubulin was isolated by selective precipitation from bovine brain homogenate by an adaptation of the method of Andreu (2007) and lyophilized by an adaptation of the method of Dráberová et al. (2010). The resulting product is >99% pure (Figure 1) and polymerization competent (Figure 2). Lyophilized Tubulin is supplied as a white powder with a tubulin-to-powder weight ratio of 1:3. When reconstituted with ultrapure water to 40 mg/ml, the buffer conditions are 10 mM Sodium Phosphate, 0.5 mM MgCl2, 0.1 mM GTP, and 0.25 M Trehalose, pH 7.0.
When supplemented with guanosine (GTP or GMPCPP) and warmed to 37°C, Lyophilized Tubulin will polymerize into MTs when above its critical concentration. The recommended tubulin concentration for ensuring polymerization is 2 mg/ml.
Lyophilized Tubulin is supplied for use in cell-free experimental systems including:
- structural analysis by X-ray crystallography and electron microscopy
- drug discovery by high-throughput screening
- in vitro biochemical and biophysical approaches
Greater purity at a better price.
Figure 2: Lyophilized Tubulin is polymerization-competent. Absorbance (340 nm) of Lyophilized Tubulin at 5 mg/ml in Tubulin PEM Buffer (Cat. No. 032002; 80 mM PIPES, 1 mM EGTA, and 1 mM MgCl2, pH 6.8) supplemented with 1 mM GTP, 1 mM DTT, and 5% Glycerol at at 37°C. Distinct nucleation and polymerization phases are evident.