Spindle-F-Actin, Molecular Gearshifts, and Cytoplasmic Softening
Spindle-F-Actin Interactions in Mitotic Spindles in an Intact Vertebrate Epithelium
Kita et al. | Molecular Biology of the Cell A compelling subject for striking microscopy images, microtubules have long been considered the major mechanical component of the mitotic spindle. But a long-standing question has been whether a second cytoskeletal system lurks beneath the surface. In this study, the Bement lab develops improved methods for preserving F-actin in embryonic epithelia and successfully visualizes F-actin associated with the mitotic spindle. The authors demonstrate that, like spindle microtubules, multiple populations of spindle-F-actin exist and reorganize as a function of the cell cycle. By providing some of the first evidence that F-actin is a component of the mitotic spindle in animal cells, this study challenges the view that F-actin is restricted to the cell periphery during cell division.
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Enhancements to a proximity-based labeling approach allow mapping of cell-cycle-dependent structural proteins.
Remnant et al. | Molecular Biology of the Cell
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