Journal Club – July 2020

Cytoskeleton Storms, Polarized Actin Cortex, and Protein Liquids

 

Featured


Membrane-Proximal F-Actin Restricts Local Membrane Protrusions and Directs Cell Migration

Bisaria et al.  |  Meyer Lab  |  Science

Global F-actin concentrations are highest at the front of migrating cells where actin-based protrusions push the membrane forward. However, subpopulations of F-actin may be performing opposing functions to achieve directed cell migration. Here, the Meyer lab develops a probe that selectively illuminates the cell cortex in order to visualize membrane-proximal actin (MPA) independently of actin protrusions. The authors discover that MPA levels are lowest toward the front of the cell despite an overall enrichment of F-actin in this region. Polarized MPA density likely directs cell migration by regulating membrane tension.

 

Video


cytoskeleton storm
 
The Gatlin Lab captures a stunning video of a “cytoskeleton storm” using microfluidics and photo-patterning.

Watch the video at the ASCB GFP image and video contest site.

Read the publication utilizing this methodology in the Journal Club Picks section below.

 
 
 
 

Journal Club Picks (find us in the methods section)


  • Microtubule growth slows in response to nearby growing plus ends.
    Geisterfer et al.  | Gatlin Lab  |  Current Biology
  • An adaptor protein synchronizes spindle disassembly and nuclear envelope reassembly.
    von Appen et al.  | Ullman and Frost Labs  |  Nature
  • A 3-protein module simultaneously grows single microtubules while stalling bundled microtubules.
    Mani, Jiang, and Subramanian  | Subramanian Lab  |  bioRxiv
  • A microtubule stabilizing compound has anti-cancer activity in taxol-resistant cancer models.
    Guo et al.  | McDaid Lab  |  Molecular Pharmacology
  • The chromosomal passenger complex hitches a ride with MKLP2 during anaphase.
    Adriaans et al.  | Akhmanova and Lens Labs  |  Current Biology
  • Cryo-EM structures reveal modular subcomplexes within the native human γ-TuRC.
    Wieczorek et al.  | Kapoor Lab  |  Cell Reports
  • Actin spontaneously partitions into droplets to form protein liquids.
    Scheff et al.  | Gardel Lab  |  Soft Matter
  • Cycles of dynamin assembly and disassembly generate stiff actin super-bundles.
    Zhang et al.  | Chen Lab  |  Nature Cell Biology
  • Actin monomer distribution regulates the assembly of complex actin superstructures.
    Skruber et al.  | Vitriol Lab  |  Current Biology

 

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