Journal Club – January 2020

Expert Protocols, Microtubule Branching, and the Actin Interactome

Must Read

Cytoskeleton Dynamics: Methods and Protocols

Helder Maiato (Ed.)

Cytoskeleton Dynamics

This Methods in Molecular Biology series outlines a range of experimental approaches for studying actin and microtubule dynamics, and is sure to be an invaluable resource to new and seasoned researchers alike. View the Table of Contents.

  • reconstitution of dynamic systems
  • studying protein-protein interactions
  • fluorescence and structural microscopy

Journal Club Picks

  • Selective destabilization of dynamic microtubules limits synaptic bouton growth.
    Coombes et al.  |  Wildonger and Gardner Labs  |  Current Biology
  • Disruption of the kinesin KIF3B causes schizophrenia‐like phenotypes in mice.
    Alsabban et al.  |  Hirokawa Lab  |  EMBO Journal
  • Kinesin motors diffusing within a 2D supported lipid bilayar accumulate on microtubules.
    Jiang et al.  |  Hancock Lab  |  Proceedings of the National Academy of Sciences
  • A minimal reconstitution system yields mechanical insights into microtubule branching.
    Alfaro-Aco et al.  |  Petry Lab  |  eLife – Find us in the methods section!
  • Full-length Ska and Ndc80 complex together in the absence of microtubules and tension.
    Huis In ‘t Veld et al.  |  Musacchio and Dogterom Labs  |  eLife
  • Profiling actin binding proteins in interphase vs. mitosis reveals key players in cell rounding.
    Serres et al.  |  Roux and Paluch Labs  |  Developmental Cell
  • Actin protrusions maintain cell–cell adhesions in confluent epithelial sheets.
    Li et al.  |  Brieher Lab  |  Proceedings of the National Academy of Sciences
  • Formin-mediated actin polymerization is regulated by the acetylation of key lysine residues.
    Mu et al.  |  Higgs Lab  |  Proceedings of the National Academy of Sciences
  • Epithelial cells transition from short/squat to tall/skinny by modulating their actin network.
    Wang and Brieher  |  Brieher Lab  |  Journal of Cell Biology

Our opinions are our own and do not represent the views or endorsement of the authors cited.

Recent Newsletters

Journal Club Archives

Latest Newsletters from Journal Club

Journal Club – June 2023

Featured Articles

Makaros et al. Mol. Cell. 2023
A study by Makaros et al. explores the role of C-terminal degrons in ubiquitin-independent proteasomal degradation. The study suggests that this mechanism may be crucial in coping with aberrant proteins accumulated in response to cellular stress. The conservation of C-degron-mediated degradation from bacteria to humans is also discussed, highlighting its evolutionary significance.

Read More »

Journal Club – August 2020

WAVE-Deficient Autoimmune Disorders, Wandering Monopolar Spindles, and a Guide to Selecting Cellular Actin Probes Journal Club Picks Five patients with immunodeficiency harbor mutations in the WAVE regulatory complex.Cook et al.

Read More »

Journal Club – July 2020

Cytoskeleton Storms, Polarized Actin Cortex, and Protein Liquids Featured Membrane-Proximal F-Actin Restricts Local Membrane Protrusions and Directs Cell Migration Bisaria et al.  |  Meyer Lab  |  ScienceGlobal F-actin concentrations are

Read More »

Journal Club – May 2020

Spiraling Kinesins, Small Molecule Degraders, and Foldable Actin Networks Featured Kinesin-14 Motors Drive a Right-Handed Helical Motion of Antiparallel Microtubules Around Each Other Mitra et al.  |  Diez Lab  |

Read More »
Scroll to Top