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|032003||5 x 1 ml|
Tubulin PEM Buffer (5X) is comprised of 400 mM PIPES, 5 mM EGTA, and 5 mM MgCl2 (pH = 6.8 with KOH).
Tubulin PEM Buffer (5X) is stable at 4°C for 1 year.
Tubulin PEM Buffer (5X) supports the stability and activity of purified tubulin in vitro when diluted to 1X and supplemented with 1 mM each DTT and guanosine (GTP or GMPCPP).