logo_tagline
Address_600_150

.

DATASHEET: TUBULIN PEM BUFFER

Catalog Number: 032002
Store at 4°C
Material:

Tubulin PEM Buffer is comprised of 80 mM PIPES, 1 mM EGTA, and 1 mM MgCl2 (pH 6.8 with KOH).

Storage and Handling:

Tubulin PEM Buffer is stable at 4°C for 1 year.

Uses:

Tubulin PEM Buffer supports the stability and activity of purified tubulin in vitro when supplemented with 1 mM each DTT and guanosine (GTP or GMPCPP).

Polymerization Protocol:

Dilute Lyophilized Tubulin (Cat. No. 142001) or Cycled Tubulin™ (Cat. No. 032005) to 2 mg/ml with Tubulin PEM Buffer and supplement with 1 mM each DTT and guanosine (GTP or GMPCPP). Incubate on ice for 5 minutes, then transfer to a 37°C water bath for 1 hour. If polymerized with GMPCPP or protected with Taxol, the resulting MTs will be stable at room temperature for several days. Do not place polymerized MTs on ice.


Download as PDF