Datasheet
Actin Polymer Buffer 10X
| Catalog Number | Volume |
|---|---|
| 000103 | 5 x 1 ml |
Store at 4°C and protect from light
For research use only. Not for medical or diagnostic use.
Material
Actin Polymer Buffer (10X) is comprised of 500 mM KCl and 20 mM MgCl2.
Storage and Handling
Actin Polymer Buffer (10X) is stable at 4°C for 1 year.
Activity and Applications
Actin Polymer Buffer (10X) supports the polymerization of G-actin into F-actin in vitro.
Polymerization Protocol
Dilute Ultra-Pure Actin (Cat. No. 160101) or Pure Actin (Cat. No. 190101) to 0.4 mg/ml with Actin Working Buffer (Cat. No. 000102; 5 mM Tris-HCl and 0.2 mM CaCl2, pH 8.0) supplemented with 0.2 mM ATP and 1 mM DTT. Incubate on ice for 1 hour followed by centrifugation at 14k rpm for 15 minutes at 4°C. Collect the supernatant and add 1/10 volume of Actin Polymer Buffer (10X) and ATP to 1 mM. Incubate at room temperature for 1 hour to polymerize. The resulting actin filaments can be stored at 4°C for 2 weeks with the addition of 0.05% sodium azide.
