For general, purchasing, or technical questions, email [email protected].
Copyright © 2023 PurSolutions, LLC
Catalog Number: 033305
Made in the USA
Purity: >99%
Source: bovine
Molecular Weight: ~110 kDa
Maximum Excitation/Emission:Â 494/517 nm
Labeling Stoichiometry:Â ~4.0 (check product label)
Form: clear aqueous solution
Concentration: 20 mg/ml<
Buffer Conditions: 50 mM K-Glutamate and 0.5 mM MgCl2 (pH 7.0)
Shipping: shipped on dry ice
Storage Conditions: store at -80ËšC immediately
Shelf Life: check product label for expiration date
Labeled Tubulin-Biotin-XX binds to streptavidin-coated beads. Coomassie G250-stained protein gel of unbound and bound fractions of tubulin protein samples incubated with Dynabeads® M-280 Streptavidin (Thermo Fisher 11205D). Cycled Tubulin™ (Cat. No. 032005) serves as the non-biotinylated negative control. Labeled Tubulin-Biotin-XX (Cat. No. 033305) fractionates with the streptavidin-coated beads.
Labeled Tubulin-Biotin-XX binds to streptavidin-coated coverslips. Fluorescent images of microtubules incubated in streptavidin-coated flow cells. Microtubules were polymerized with 1.8 mg/ml Cycled Tubulin™ (Cat. No. 032005) and 0.2 mg/ml Labeled Tubulin-Alexa Fluor® 647 (Cat. No. 064705) +/- 0.05 mg/ml Labeled Tubulin-Biotin-XX (Cat. No. 033305). Microtubules polymerized with Labeled Tubulin-Biotin-XX are bound to the flow-cell surface.
For general, purchasing, or technical questions, email [email protected].
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