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Catalog Number: 064705
Made in the USA
Purity: >99%
Source: bovine
Molecular Weight: ~110 kDa
Maximum Excitation/Emission:Â 651/672 nm
Labeling Stoichiometry:Â ~0.5 (check product label)
Form: blue aqueous solution
Concentration: 20 mg/ml
Buffer Conditions: 50 mM K-Glutamate and 0.5 mM MgCl2 (pH 7.0)
Shipping: shipped on dry ice
Storage Conditions: store at -80ËšC immediately
Shelf Life: check product label for expiration date
Taxol Stabilized Microtubules (long and flexible)
Taxol-stabilized microtubules at 5 mg/ml total tubulin protein with Labeled Tubulin-Alexa Fluor® 647 (Cat. No. 064705) and Cycled Tubulin™ (Cat. No. 032005) at a 1:5 ratio. Images acquired on a widefield fluorescence microscope. View protocol.
GMPCPP Stabilized Microtubules (short and rigid)
GMPCPP-stabilized microtubules at 2 mg/ml total tubulin protein with Labeled Tubulin-Alexa Fluor® 647 (Cat. No. 064705) and Cycled Tubulin™ (Cat. No. 032005) at a 1:5 ratio. Images acquired on a widefield fluorescence microscope. View protocol.
Labeled Tubulin-Alexa Fluor® 647 is generated by reacting Alexa Fluor® 647 succinimidyl ester with >99% pure tubulin protein, thereby covalently linking the dye to random tubulin surface lysines. Polymerization competency is maintained during the labeling process by reacting the dye with polymerized microtubules and subjecting the tubulin protein to a final polymerization/depolymerization cycle. The final labeled tubulin protein displays maximum absorption at 280 nm and 651 nm, and a labeling stoichiometry ([dye]/[tubulin]) of ~0.5 as determined by spectroscopic analysis (Figure 1). Specific labeling stoichiometries are indicated on the product label. Labeled Tubulin-Alexa Fluor® 647 is commonly visualized with a Cy5 filter set with maximum excitation/emission wavelengths of 651/672 nm (see images above). The product is cryopreserved at 20 mg/ml in 50 mM K-Glutamate and 0.5 mM MgCl2 (pH=7.0).
Labeled Tubulin-Alexa Fluor® 647 displays maximum absorption at 280 nm and 651 nm.
Spectroscopic analysis reveals a peak at 280 nm and 651 nm, indicating absorption by the tubulin protein and Alexa Fluor® 647 dye, respectively.
For general, purchasing, or technical questions, email [email protected].
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