Labeled Tubulin Protein - Alexa Fluor® 647

>99% pure tubulin protein conjugated with a far red fluorescent dye.

Labeled Tubulin-Alexa Fluor® 647

Catalog Number: 064705

Product Specifications:

Purity: >99%

Source: bovine

Molecular Weight: ~110 kDa

Maximum Excitation/Emission: 651/672 nm

Labeling Stoichiometry: ~0.5 (check product label)

Form: blue aqueous solution

Concentration: 20 mg/ml

Buffer Conditions: 50 mM K-Glutamate and 0.5 mM MgCl2 (pH 7.0)

Shipping: shipped on dry ice

Storage Conditions: store at -80˚C immediately

Shelf Life: check product label for expiration date

Taxol Microtubules

Taxol stabilized microtubules polymerized from Alexa Fluor 647 labeled tubulin protein

Taxol Stabilized Microtubules (long and flexible)
Taxol-stabilized microtubules at 5 mg/ml total tubulin protein with Labeled Tubulin-Alexa Fluor® 647 (Cat. No. 064705) and Cycled Tubulin™ (Cat. No. 032005) at a 1:5 ratio. Images acquired on a widefield fluorescence microscope. View protocol.

GMPCPP Microtubules

Material

Labeled Tubulin-Alexa Fluor® 647 is generated by reacting Alexa Fluor® 647 succinimidyl ester with >99% pure tubulin protein, thereby covalently linking the dye to random tubulin surface lysines. Polymerization competency is maintained during the labeling process by reacting the dye with polymerized microtubules and subjecting the tubulin protein to a final polymerization/depolymerization cycle. The final labeled tubulin protein displays maximum absorption at 280 nm and 651 nm, and a labeling stoichiometry ([dye]/[tubulin]) of ~0.5 as determined by spectroscopic analysis (Figure 1). Specific labeling stoichiometries are indicated on the product label. Labeled Tubulin-Alexa Fluor® 647 is commonly visualized with a Cy5 filter set with maximum excitation/emission wavelengths of 651/672 nm (see images above). The product is cryopreserved at 20 mg/ml in 50 mM K-Glutamate and 0.5 mM MgCl2 (pH=7.0).

Activity and Applications

Labeled Tubulin-Alexa Fluor® 647 will polymerize into microtubules when supplemented with guanosine-5'-triphosphate (GTP), warmed to 37˚C, and kept above its critical concentration. Polymerization activity is detectable in a variety of experimental systems including fluorescence microscopy assays, turbidity assays, and GTPase assays. Labeled tubulin protein is suitable for use in a variety of fluorescent experimental applications including live cell injection, and can be combined with unlabeled tubulin protein (Cycled Tubulin™ highly recommended; Cat. No. 032005) in generating fluorescent microtubules in vitro. Visit our protocols page for common microtubule polymerization protocols, including the generation of short, rigid microtubules stabilized by GMPCPP or long, flexible microtubules stabilized by taxol.

  • in vitro microtubule gliding assays
  • single molecule kinesin and dynein motor assays
  • speckle microscopy
  • live cell injection
  • in vitro nanoscale devices
  • TIRF, STORM, SIM, STED, TPE, confocal, and widefield microscopy

The Brightest Far Red Tubulin Protein On The Market

Figure 1:

Labeled Tubulin-Alexa Fluor® 647 displays maximum absorption at 280 nm and 651 nm. Spectroscopic analysis reveals a peak at 280 nm and 651 nm, indicating absorption by the tubulin protein and Alexa Fluor® 647 dye, respectively.

ALEXA FLUOR® 647 - Absorbance Graph