Actin Pelleting Assay

Actin pelleting assay graphical abstract


At relatively high centripetal forces, filamentous F-actin will pellet while globular G-actin will remain suspended in solution. This biophysical property can be leveraged in probing for actin-associated proteins and assessing actin polymerization competency. This protocol outlines how to fractionate actin protein solutions by centrifugation and probe the resulting pellet and supernatant fractions.